Product Name VITAMIN K1（PHYTONADIONE）
CAS No. 84-80-0
Appearance Yellow to orange clarified viscous liquid; odorless or almost odorless; easy to decompose when exposed to light.soluble in chloroform, ether or vegetable oil, slightly soluble in ethanol and insoluble in water.
Isomer content % ≤21
Refractive index 1.52-1.53
Identification
(1) Infrared Spectroscopy:197F (CN 1-May- 2020)
(2)Ultraviolet-Visible Spectroscopy:197U (CN 1- May-2020) . Absorptivities do not differ by more than 3.0%
Limit of Menadione No purple or blue color is produced
Menadione
Take 20mg of this product, add 2mL of trimethylpentane to dissolve, add ammonia test solution-ethanol (1:1) lmL and 2 drops of ethyl cyanoacetate, shake gently, after placing，the following layer of solution appears blue, Comparing with the trimethylpentane solution (containing 20 ug/mL of Menadione reference substance) 2 mL prepared by the same method, it should not be deeper.The test result less than 0.2%
Total Aerobic Microbial Colonies Count CFU/g ≤1000
Yeasts & Molds CFU/g ≤50
Vitamin K1 % 97—103
Conclusion The product comply with USP 2021
